由于環(huán)境因素和細(xì)胞內(nèi)的正常代謝過程造成的DNA損傷，每個(gè)細(xì)胞每天都會(huì)發(fā)生1,000到1,000,000個(gè)。雖然這些只占人類基因組約60億個(gè)堿基中的一小部分，但如果關(guān)鍵基因損傷未及時(shí)修復(fù)，可能會(huì)阻礙細(xì)胞的正常生理功能，進(jìn)而增加癌變可能。彗星實(shí)驗(yàn)，或稱單細(xì)胞凝膠電泳（Single cell gel electrophoresis，SCGE），是一種測(cè)量單個(gè)細(xì)胞DNA損傷的常用技術(shù)。其原理很簡單，即在電泳場(chǎng)中，將受損細(xì)胞DNA（包含片段和鏈斷裂）與完整的DNA分離，通過顯微鏡可觀察到損傷細(xì)胞呈現(xiàn)出典型的彗星狀尾巴，然后通過測(cè)量計(jì)算彗尾大小對(duì)比出細(xì)胞DNA損傷的程度。因?yàn)?/span>彗星實(shí)驗(yàn)的特點(diǎn)，該方法幾乎被用來評(píng)估任何類型的真核細(xì)胞的 DNA 修復(fù)能力，包括雙、單鏈斷裂的不同的 DNA 損傷情況。是一種能快速、大通量檢測(cè)真核細(xì)胞DNA損傷進(jìn)而判別遺傳毒性的技術(shù)。

彗星實(shí)驗(yàn)結(jié)果圖

彗星實(shí)驗(yàn)原理雖簡單，但操作繁瑣，需要豐富的實(shí)驗(yàn)經(jīng)驗(yàn)和技巧，尤其常常出現(xiàn)的“脫膠”問題，困擾了許多科研人員。除此之外，有時(shí)為了跑出完美的“彗星”圖案放在paper里，還需要重復(fù)做許多次實(shí)驗(yàn)，費(fèi)時(shí)費(fèi)力。為了解決上述問題，我們推薦CellBiolabs的OxiSelectTMComet Assay Kit即彗星實(shí)驗(yàn)試劑盒來檢測(cè)細(xì)胞的DNA損傷。該試劑盒不僅能讓彗星實(shí)驗(yàn)化繁為簡，還有兩種不同規(guī)格（3孔和96孔）的細(xì)胞電泳凝膠板供選擇，讓少量樣本和大量樣本的DNA損傷檢測(cè)通通輕松hold住。用該試劑盒做彗星實(shí)驗(yàn)流程如下圖。

除了操作簡便，OxiSelectTMComet Assay Kit還有以下優(yōu)點(diǎn)：

1)      適用于各種DNA損傷檢測(cè)，是一款非常好用的DNA損傷檢測(cè)篩選工具；

2)      試劑盒中的載玻片經(jīng)過特殊處理以粘附低熔點(diǎn)瓊脂糖，避免“脫膠”問題出現(xiàn)；

3)      采用特殊的DNA熒光染料，能有效降低背景干擾，更加方便讀取實(shí)驗(yàn)結(jié)果。

彗星實(shí)驗(yàn)試劑盒信息：

為了滿足客戶更多樣的實(shí)驗(yàn)需求，彗星實(shí)驗(yàn)試劑盒內(nèi)特殊處理電泳載玻片還可以單獨(dú)購買，詳情如下：

產(chǎn)品部分發(fā)表文獻(xiàn)：

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